Rapid and selective aptamer-based fluorescence detection of salivary lysozyme using plasmonic metal-enhanced fluorescence of ZnSSe alloyed quantum dots-gold nanoparticle nanohybrid

• Anti-lysozyme DNA aptamer (Apt) was immobilised on L -cysteine ZnSSe quantum dots (QDs). Plasmonic gold nanoparticles (AuNPs) were bonded to the Apt-ZnSSe QDs. Lysozyme quantitatively detected by QDs-AuNPs biosensor probe. The highly selective against tested enzymes. successfully in human saliva. We report construction of an (Apt)-based fluorescent platform for salivary lysozyme using plasmonic metal-enhanced fluorescence alloyed (QDs)-gold nanoparticle (AuNP) nanohybrid. Salivary used as a model analyte develop Apt assay real-life detection To construct biosensor, thiolated anti-salivary -cysteine-ZnSSe QDs surface form functionalised Apt-ZnSSe-QDs. Thereafter, citrate-AuNPs Apt-ZnSSe-QDs Apt-ZnSSe-QDs-AuNP Using affinity-based biomolecular binding interaction between and target lysozyme, effect induced localised plasmon resonance (LSPR) signal from AuNPs amplify upon recognition. Probing LSPR amplifying effects different NPs, our analysis showed that spherical size 6 nm most effective Under optimum experimental reaction conditions, within 5 min with limit 22.9 ?g/mL other Application QDs-AuNP probe detect real saliva samples achieved.